R@CMon hosted Australia’s first Ceph Day

Ceph Days are a series of regular events in support of the Ceph open source community. They now occur at locations all around the world. In November, R@CMon hosted Australia’s first Ceph Day. The day hosted 70-odd guests, many of which were from interstate and a few from overseas. There participants were from the research sector, private industry and ICT providers. It was a fantastic culmination of Australia’s growing Ceph community.

If you don’t already know, Ceph is basically an open-source technology for software-defined cluster-based storage. It means our storage backend is essentially infinitely scalable, and our focus can shift to the access mechanisms for data.

Check out the promo:

[youtube https://www.youtube.com/watch?v=vcK6KSA0DN0&w=500&h=281]

R@CMon has pioneered the adoption of Ceph for accessible research data storage and at mid-2013 was the first NeCTAR Research Cloud node to provide un-throttled volume storage. R@CMon has also worked closely with was InkTank and now Redhat to develop the support model for such an enterprise (see Ceph Enterprise – a disruptive period in the storage marketplace).

The day began with the Ceph Community Director – Patrick McGarry. His presentation included information about the upcoming expanded Ceph metrics platform, what the Ceph User Committee has been up to, new community infrastructure for a better contributor experience, and revised open source governance.

[youtube https://www.youtube.com/watch?v=joCp3WByV9E&w=500&h=281]

Undoubtedly the highlight of the day was the joint talk given by R@CMon’s very own director – Steve Quenette and technical lead – Blair Bethwaite. Here we explain Ceph in the context of the 21st century microscope – the tool each researcher creates to do modern day research. We also explain how we technically approached creating our fabric.

[youtube https://www.youtube.com/watch?v=aZNwQieDpfg&w=500&h=281]

R@CMon announced as a Mellanox “HPC Center of Excellence”

At SuperComputing 2015 in Austin our network/fabric partner Mellanox announced R@CMon (Monash University) as a “HPC Centre of Excellence“. A core goal of the HPC CoE is to drive the technological innovations required for the next generation (exascale) supercomputing, whilst also ensuring that such an exascale computer is relevant to modern research. R@CMon is a stand out pioneer at converging cloud, HPC and data, all of which are key to the “next generation”.

“We see Monash as a leader in Cloud and HPC on the Cloud with Openstack, Ceph and Lustre on our Ethernet CloudX platform.” Sudarshan Ramachandran, Regional Sales Manager, Australia & New Zealand

From a fabric innovation point of view, it has been a very productive and exciting 24months for R@CMon. By early 2014 the internal Monash University HPC system “MCC” was burst onto the Research Cloud, allowing a researcher’s own merit the be leveraged with institutional investment. It also represents a shift towards soft HPC, where the size of a HPC system changes regularly with time. Earlier this year we announced our early adoption of RoCE (RDMA over Converged Ethernet) using Mellanox technologies. The meant the same fabric used for cloud networking could also be used for HPC and data storage backplanes. In turn MCC on the R@CMon also enabled RDMA communications, that is, real HPC performance but on an otherwise orchestrated cloud.

Finally at the Tokyo OpenSack summit 2015, Mellanox announced R@CMon as debuting the World’s first 100G End-to-End Cloud. This technology eases scaling and heterogeneity of performance aspects. In particular, it sets the basis for processor and storage performance for peak and converged cloud/HPC needs. Watch this space!

The Ramialison Group Analysis Workflow on R@CMon

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The Ramialison Group at the Australian Regenerative Medicine Institute (ARMI) located in the biomedical research precinct of Monash University, Clayton specialises in systems biology both on the bench and through computational analysis. Their work is driven by the in vivo and in silico dissection of regulatory mechanisms involved in heart development, where deregulation of such mechanisms cause congenital heart disease, which results in 1 out of every 100 babies to be born with heart defects in Australia.

Heatmap generated from transcriptomic data from heart samples (Nathalia Tan)

Their research focuses on identifying DNA elements that play a crucial role in the development of the heart and, that could be impaired in disease. To identify these sequences, several genome-wide interrogation technologies (genomics and transcriptomics) are employed on different model organisms such as mouse or zebrafish. Downstream analysis of the data generated from these experiments involves high performance computing and requires large storage, which can be up to hundreds of gigabytes in size for a single project.

To optimise their investigation into heart development, the R@CMon team has deployed a dedicated Decoding Heart Development and Disease (DHDD) server on the Monash node of the NeCTAR Research Cloud infrastructure, which has now been running for over a year. This has not only provided the group with faster processing speeds in comparison to running jobs on a local desktop, but also an appropriate file storage infrastructure with persistent storage for files that are regularly accessed during analysis. Through VicNode, the group has been given vault storage for archiving completed results for their various research projects. With the assistance R@CMon, the group has been able to easily add users to the server as it continues to grow with new members and local collaborators.

Web interface for the Trawler web service.

In addition to the DHDD server, the R@CMon team also assisted the Ramialison Group in deploying a dedicated cloud server that has been used to develop the Trawler motif discovery tool web service. The implementation of this tool allows the group to quickly and easily analyse next-generation sequencing data and identify overrepresented motifs, which has led to a manuscript that is currently in preparation. The Ramialison Group envisage future developments of similar simple and easy to use bioinformatics analysis tools through R@CMon.

R@CMon Storage

Our journey towards R@CMon Storage (Storage-as-a-Service)…

In May 2013 R@CMon went live with an OpenStack cell within the NeCTAR (Australian) Research Cloud confederation. It was an innovation in its own right, targeting the commodity end of both the fundamental and translational research needs of Australia (see R@CMon IDC Spotlight – AMD & DELL). Our technical partner, Dell, has successfully applied the design pattern to many other subsequent Research Cloud nodes, and many other OpenStack based private cloud deployments both nationally and internationally. Shortly after the launch of this initial IaaS compute cell, we introduced Ceph based volume storage, becoming the first volume storage service on the Research Cloud, and in doing so, instigated a collaboration with InkTank (now Redhat). By November 2014 R@CMon launched the “Phase 2” Specialist IaaS cell, an “e”-resource motivated by research that pushes boundaries. Within this cell R@CMon added an RDMA-able interconnect to our storage and compute fabric, instigating an innovative technical collaboration with Mellanox.

Thus R@CMon is an environment to build what we call “21st Century Microscopes” – where researchers orchestrate the instruments, compute, storage, analysis and visualisation themselves, looking down and tuning this 21st century lens, using big data and big computing to make new discoveries.

And accordingly, R@CMon is an environment for innovative data services for the long-tail (if you like – more ICT like). Unashamedly – Our instances of Ceph is what we can “enterprise”, whilst each user or tenant has their own needs on file protocol, capacity and latency.

R@CMon Storage is a collection of storage access methods and underlying storage infrastructure products. Why do we present storage as both front-ends and infrastructure? Because most users want access methods – it should just work, but most microscope builders want infrastructure – it should be a building block. R@CMon Storage is also the Monash operating centre to VicNode – where we explain some of these products.

We now have a series of R@CMon Storage products and services available – ranging from infrastructure products, access methods and data management.

Histone H3.3 Analysis on R@CMon

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The Epigenetics and Chromatin (EpiC) Lab at Monash University is working on understanding how mutations in certain chromatin factors promote the formation of brain tumours. This project involves the generation and analysis of high-throughput sequencing data of chromatin modifications and remodellers in normal and mutated cells. The sequencing is carried out at the MHTP Medical Genomics Facility and the resulting datasets are then imported into the analysis workflow running on the Monash node (R@CMon) of the NeCTAR Research Cloud. The sequencing reads are first aligned to the repetitive fraction of the genome using a script developed by Day et al. (Genome Biology 2010) to determine enrichment at repeats. Sequencing reads are then aligned to the genome using Bowtie. The resulting files are filtered for quality, poor matches and PCR duplicates using customised Perl scripts. The filtered files are then imported into SeqMonk for further analysis.

Overlap analysis using SeqMonk

This allows for rapid visualisation of individual aligned reads across the entire genome. The inbuilt MACs peak caller is used for first pass peak calling. A selection of peaks is then validated in the lab by ChIP-qPCR experiments and peak-calling parameters can be adjusted based on these results. Overlap analysis with regions of interest can be performed in SeqMonk. Aligned sequence files are converted to BigWig format using customised Perl scripts and uploaded onto the NeCTAR Object Storage (Swift), which can then be loaded seamlessly on the UCSC Genome Browser for visualisation and further investigation. Once the sequence files are uploaded to the object storage, it can then be easily compared against public ENCODE datasets and UCSC genomic annotations to identify any potentially interesting correlations.

Aligned sequence visualisation using the UCSC Genome Browser.

The R@CMon team and the Monash Bioinformatics Platform supported the EpiC Lab by deploying a dedicated analysis instance on the NeCTAR Research Cloud based on the training environment first developed for the BPA-CSIRO Bioinformatics Training Platform. The open access and reusability of the training platform means it can be easily readapted to various analysis workflows. The R@CMon team and the Monash Bioinformatics Platform will continue to engage with the EpiC Lab as they grow and scale their analysis workflow on the NeCTAR Research Cloud.